Chamber Cleaner is a surface disinfectant for use inside anaerobic chambers or glove boxes. Chamber Cleaner is a 0.133% solution of Benzalkonium chloride. Benzalkonium chloride is a quaternary ammonium compound and cationic surfactant. The disinfectant action is based on increased membrane permeability.

 

View the full product insert: Zephrin.pdf

Zephrin Products

 

Item #	Description	Size	Price
AS-972	Zephrin Chamber Cleaner	1 gal	17.25

 

Formula

Benzalkonium Chloride, 1.33 mL

Distilled Water, 1000.0 mL 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Wear suitable protective clothing including gloves and eye/face protection.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal. 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C -27°C) until used.  Avoid overheating or freezing.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  1 year from date of manufacture. 

Procedure

Methods for Use:  The Chamber Cleaner container should be thoroughly mixed by inverting the closed container.  Quantities suitable for daily use in the chamber should be dispensed into smaller squeeze bottles.  A generous quantity should be applied onto the area to be cleaned.  Using a paper towel, the liquid should be wiped over the desired area.  The area to be cleaned should be left wet with chamber cleaner.  Do not completely wipe up or dry the area to be cleaned.  Leave wet and allow to air dry.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

Materials Required But Not Provided

Standard microbiological supplies and equipment such as paper towels, gloves, and appropriate biohazard disposal containers.

Limitations

Chamber Cleaner is not a sterilizing agent.  Consult reference materials for additional information (6).

Quality Control

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

 

Physical Appearance: Chamber Cleaner should appear as a clear, colorless liquid. 

References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974.  Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory Manual.  USDHEW C. D. C. Atlanta, GA 30333.

 

2.        Dowell, V. R., Jr., G. L. Lombard, F. S. Thompson and A. Y. Armfiedl.  1977.  Media for the Isolation, Characterization, and Identification of Obligately Anaerobic Bacteria.  USDHEW, CDC, Atlanta, GA 30333.

 

3.        Holdeman, L. V., F. P. Cato and W. E. C. Moore.  1977.  Anaerobe Laboratory Manual.  Virginia Polytechnic Institute and State University.  Blacksburg, VA 24061.

 

4.        Jousimies-Somer, H. R., P. Summanen, D. M. Citron, E. J. Baron, H. M. Wexler and S. M. Finegold.  2002.  Wadsworth – KTL Anaerobic Bacteriology Manual.  Star Publishing Co., Belmont, CA 94002.

 

5.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

 

6.        Merianos, J.J.  1991.  Quaternary ammonium antimicrobial compounds. In S.S. Block (ed.), Disinfection, Sterilization and Preservation, 4^th ed. (pp. 225-255).  Lea Febiger, Malvern, PA.