AS-424 combination package is the standard primary setup for anaerobic culture workup.  This combination contains a Brucella Blood Agar (BRU), Phenylethylalcohol Blood Agar, Laked Brucella Blood Agar (LBA) and a Bacteroides Bile Esculin Agar (BBE)/Laked Kanamycin Vancomycin (LKV) biplate.  This convenient four plate package contains all the necessary media types in one easy to open package.  All our media is pre-reduced and anaerobically sterilized (PRAS) so all you have to do is streak the plates.  With a three month shelf-life and room temperature storage this is the most convenient system available.

BRU is an enriched non-selective agar medium. BRU is intended for the isolation, quantitation and partial identification of obligate anaerobic bacteria from clinical specimens.  It is supplemented with vitamin K₁ and hemin to facilitate the recovery and pigment production of Prevotella melaninogenica.  Sheep blood has been added for the observation of hemolytic reactions as seen by the double zone b - hemolysis of Clostridium perfringens.  This medium will also support the growth of aerobic and microaerophilic bacteria if incubated appropriately.  BRU is also suitable for use in susceptibility testing, antibiotic differential disk examination and spot biochemical testing.  This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

BBE is an enriched selective and differential medium for the isolation and presumptive identification of obligately anaerobic gram-negative bacilli of the Bacteroides fragilis group. BBE contains gentamicin at a concentration which inhibits most facultative anaerobes. This medium also contains bile, which inhibits anaerobic gram-negative rods except the B. fragilis group and other bile resistant Bacteroides and Fusobacteria. Esculin in the medium permits recognition of esculin hydrolysis by organisms that produce a brown to black coloration in the medium around the colonies. Hemin is added as a growth factor and allows testing for catalase production. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

LKV is an enriched selective medium for the isolation and partial identification of obligately anaerobic gram-negative bacilli.  LKV contains vancomycin to inhibit the growth of gram-positive microorganisms and kanamycin to inhibit the growth of gram-negative, facultatively-anaerobic bacilli.  The sheep blood in the medium is laked and vitamin K₁ is added to facilitate the recovery and pigment production of Prevotella melaninogenica.  This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

PEA is an enriched selective medium for the isolation of obligately anaerobic bacteria from clinical specimens. PEA medium contains phenylethylalcohol to inhibit the growth of gram-negative, facultatively-anaerobic bacteria and prevent swarming. This medium will support the growth of most obligate anaerobic bacteria, both gram positive and gram negative. The PEA medium is especially useful in selective isolation of anaerobes from mixed populations that contain rapidly growing gram-negative bacteria such as Proteus species. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

LBA is intended for the isolation, quantitation and partial identification of obligately anaerobic bacteria from clinical specimens. This medium will also support the growth of aerobic and microaerophilic bacteria if incubated appropriately, an enriched non-selective medium. It is supplemented with vitamin K₁, hemin and laked sheep blood to facilitate the recovery and pigment production of Prevotella and Porphyromonas. This medium is prepared, stored, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use. 

     Anaerobe Systems PRAS BRU PEA LBA BBE/LKV AS-424

View the full product insert for each media component:  BRU Insert.pdf,  PEA Insert.pdf,