Cadmium Sulfate Fluoride Acridine Trypticase Agar (CFAT) has been formulated for the enhanced isolation of Actinomyces naeslundii and Actinomyces viscosus from mixed culture. Tryptic Soy and dextrose form the nutritive base of this medium, which is supplemented with cadmium sulfate, sodium flouride and neutral acriflavine to enhance the isolation of A. naeslundii and A. viscosus.  Colonies of Actinomyces spp.are cream to slightly greenish in color, entire, convex or raised and opaque on this medium.  This medium is prepared, dispensed and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Anaerobe Systems PRAS Cadmium Sulfate Fluoride

       Acridine Trypticase Agar (CFAT) AS-6424

Formula

Tryptic Soy Broth, 30.0 g

Dextrose, 5.0 g

Agar, 15.0 g

Cadmium Sulfate, 0.013 g

Sodium Flouride, 0.08 g

Neutral Acriflavine, 0.0012 g

Basic Fuchsin, 0.00025 g

Distilled Water, 1000.0 ml

 

Final pH 7.0 +/- 0.2 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original container until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  90 days from date of manufacture.

 

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use:  CFAT agar should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material.  Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37^oC for 2-7 days.  Additional periods of incubation may be necessary to recover some anaerobes.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

 

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

 

Limitations

CFAT agar will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar also be inoculated from clinical specimens to assure growth of all species present.  Consult reference materials for additional information.

 

Quality Control

This medium should support good growth of some Actinomyces spp. found in clinical infections.

The following organisms are routinely used for quality control testing at Anaerobe Systems. 

Organism Tested	ATCC #	Results	Time (Hours)
Fusobacterium nucleatum	25586	No Growth
Actinomyces viscosus	43146	Growth	48 hrs
Bacteroides fragilis	25285	Growth	48 hrs
Enterococcus faecalis	29212	Growth	48 hrs

 

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

 

Organism	Expected Growth
A. viscosus	48 hrs
F. nucleatum	Inhibited
B. fragilis	48 hrs

 

Physical Appearance: CFAT should appear opaque maroon red in color.

 

References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974.  Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory Manual.  USDHEW C. D. C. Atlanta, GA 30333.

 

2.        Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R.  1992.  Principles of Practices of Clinical Anaerobic Bacteriology.  Star Publishing Co., Belmont, CA 94002.

 

3.        Holdeman, L. V., F. P. Cato and W. E. C. Moore.  1977.  Anaerobe Laboratory Manual.  Virginia Polytechnic Institute and State University.  Blacksburg, VA 24061.

 

4.        Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold.  2002.  Wadsworth – KTL Anaerobic Bacteriology Manual.  Star Publishing Co.  Belmont, CA 94002.

 

5.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.