Formula

Pancreatic Digest of Casein, 10.0 g

Soy Peptone, 3.0 g

Animal Tissue, 10.0 g

Yeast Extract, 2.0 g

Sodium Chloride, 5.0 g

Sodium Bisulfite, 0.1 g

L-Cysteine, 0.5 g

Dextrose, 1.0 g

Agar, 15.0 g

Vancomycin, 10.0 mg

Polymyxin B, 2500.0 i.u.

Trimethoprim, 5.0 mg

Laked Horse Blood, 70.0 ml

Distilled Water, 1000.0 ml

 

Final pH 7.25 +/- 0.2 at 25 degrees C.

Final weight 16.0g +/- 1.6.

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

Storage and Shelf Life

Storage:  Upon receipt, store at (13°C - 27°C) in original container until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  3 months from date of manufacture.

Procedure

Specimen Collection:  Specimens for culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of specimens.

Methods for Use:  Rectal swabs or swabs of feces specimens are used to inoculate an area approximately 1 to 1^1/4 inch in diameter on the surface of this medium.  Streak the plate for isolated colonies.  Incubate inoculated plates at 42 – 43^o C in an anaerobic jar (catalyst removed) with a microaerophilic gas mixture.  For strain specific gas mixtures, consult appropriate references. 

NOTE:  If facilities for gassing out an anaerobic jar are not available, a disposable Hydrogen-Carbon Dioxide generator may be substituted.  Not all strains of Campylobacter jejuni subsp. jejuni grow as well when generators are used, and some may not grow at all.  Campylobacter jejuni subsp. jejuni is a microaerophile, not a strict anaerobe.

Plates should be examined after 24, 48 and 72 hours of incubation.  Colonies of Campylobacter jejuni subsp. jejuni are usually detected in 24 hours.  The colonies vary from pinpoint, glossy-appearing to those which spread over the entire surface of the agar. Since Campylobacter jejuni subsp. jejuni is an oxidase positive organism, the oxidase test can be used to screen suspect colonies.

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber or anaerobic jars, other culture media and serological and biochemical reagents.

 

Limitations

CAMPY will not provide complete information for identification of bacterial isolates.  Additional biochemical test procedures from a pure culture are necessary for complete identification.  Consult appropriate reference materials for additional information.

 

Quality Control

If used properly, this medium should support good growth of Campylobacter jejuni subsp. jejuni isolated from clinical materials.  Normal bowel flora organisms such as Proteus mirabilis, Escherichia coli, Enterococci, and Clostridium perfringens should be inhibited.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. 

Organism Tested	Results	Time (Hours)
Campylobacter jejuni subsp. jejuni (ATCC 33291)**	Growth	24 – 48 h
Campylobacter fetus subsp. fetus (ATCC 33246)	Growth	24 – 48 h
Staphylococcus aureus (ATCC 25923)	No Growth	24 – 48 h
Enterococcus faecalis (ATCC 29212)	No Growth
Escherichia coli (ATCC 25922)**	No Growth
Proteus mirabilis (ATCC 12453)	Inhibited to No Growth

                         ** Organisms specified by NCCLS for quality control testing of CAMPY

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

 

Organism	Expected Growth
C. jejuni subsp. jejuni	48 hrs
C. fetus subsp. fetus	48 hrs
E. coli	Inhibited
S. aureus	Inhibited

 

Physical Appearance:  CAMPY should appear opaque to translucent bright red in color.

References

1.        Morbidity and Mortality Weekly Report.  Waterborne Campylobacter Gastroenteritis – Vermont.  Vol. 24, No. 25, June 23, 1978, pg. 207.

 

2.        King, E. O.  Human infections with Vibrio fetus and a closely related Vibrio.

 

3.        Butzler, J. P., P. Dekeyser, M. Detrain and F. Dhaen.  Related vibrio in stools.  J. Ped 52:493-495, 1973.

 

4.        Skirrow, M. R.  Campylobacter enteritis:  a “new disease.  Br. Med J 2: 9-11, 1977.

 

5.        Campylobacter enteritis.  The Lancet, ii: 135-136, 1978.

 

6.        Morbidity and Mortality Weekly Report.  Campylobacter enteritis – Colorado.  Vol. 27, No. 27, July 7, 1978, pg. 226.

 

7.        Anaerobe Laboratory Manual. VPI Anaerobe Laboratory 4^th ed, Southern Printing Co., Blacksburg, VA 1977.

 

8.        Butzler, J. P., et al.  Susceptibility of related vibrios and Vibrio fetus to twelve antibiotics.  Antimicro Ag and Chemother 5: 86-89, 1974.

 

9.        Chow, A. W., et al:  Susceptibility of Campylobacter fetus to twenty-two antimicrobial agents.  Antimicro Ag and Chemoth 13: 416-418, 1978.

 

10.     Smibert, R. M.  The genus Campylobacter, An. Rev. Microbiol. 32: 673-709, 1978.

 

11.     George, H. A., P. S. Hoffman, R. M. Smibert and N. R. Krieg.  Improved media for growth and aerotolerance of Campylobacter fetus.  J. of Clin Micro. 8: 36-41, 1978.

 

12.     Blaser, M. J., P. Roesler, H. L. Hardesty, and W. L. Wang.  Carriage of Campylobacter by dogs and cats in Denver, Co.  CDC Vet. Pub. 11^th, Notes, Feb 1979.

 

13.     Blaser, M. J., J. Cravens, B. Powers, W. L. Wang.  Campylobacter enteritis associated with canine infection.  Lancet 2: 979-981, 1978.

 

14.     NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.