Anaerobe Systems PRAS Chocolate (CHOC) AS-214

View the full product insert: CHOC Insert.pdf

Formula

Pancreatic Digest of Casein, 10.0 g

Soy Peptone, 3.0 g

Animal Tissue, 10.0 g

Dextrose, 1.0 g

Yeast Extract, 2.0 g

Sodium Chloride, 5.0 g

Sodium Bisulfite, 0.1 g

Agar, 15.0 g

Cofactor, 10.0 ml

Sheep Blood, 45.5 ml

Distilled Water, 1000.0 ml

 

Final pH 7.0 +/- 0.2 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at 2 – 8^oC in original container until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  90 days from date of manufacture.

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of clinical specimens.

Methods for Use:  CHOC should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material.  Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an atmosphere containing 5% CO₂ and incubated at 35-37^oC for 18-48 hours.  Additional periods of incubation may be necessary to recover some organisms.  Detailed instructions for processing anaerobic/aerobic cultures can be found in the appropriate references (1, 2, 3).

 

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber or anaerobic jars, disinfectant, other culture media and serological and biochemical reagents.

 

Limitations

CHOC will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  Consult reference materials for additional information (1, 2, 3).

 

Quality Control

If used properly, this media should support good growth of most pathogens found in clinical infections.  In addition, the medium should support the growth of Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. 

Organism Tested	ATCC #	Results	Time (Hours)
Neisseria gonorrhoeae **	43069	Growth	24 – 48 hrs
Neisseria meningitidis	13090	Growth	24 – 48 hrs
Haemophilus influenzae **	10211	Growth	24 – 48 hrs
Streptococcus pneumoniae	6305	Growth	24 – 48 hrs
Staphylococcus aureus	25923	Growth	24 – 48 hrs
Escherichia coli	25922	Growth	24 – 48 hrs

** Organisms specified by NCCLS for quality control performance testing of Chocolate Agars.

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth.

 

Organism	Expected Growth
N. gonorrhoeae	24 hours
N. meningitidis	48 hours
H. influenzae	24 hours
S. pneumoniae	24 hours

 

Physical Appearance: CHOC should appear opaque and brown in color.

 

References

1.        Murray, R. P., et al.  1999.  Manual of Clinical Microbiology.  American Society for Microbiology Publishing, Washington, D.C. 20005.

 

2.        Finegold, S. M., and E. J. Baron.  1986.  Bailey and Scotts Diagnostic Microbiology.  C. V. Mosby Co., St.Louis, MO.

 

3.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.