Anaerobe Systems PRAS Fusobacterium
                Selective Agar (FSA) AS-6427

View the full product insert: FSA Product Information

Formula

Pancreatic Digest of Casein, 10.0 g

Soy Peptone, 3.0 g

Animal Tissue, 10.0 g

Dextrose, 1.0 g

Yeast Extract, 2.0 g

Sodium Chloride, 5.0 g

Sodium Bisulfite, 0.1 g

Hemin (0.1% soln), 2.5 ml

Agar, 15.0 g

Sodium Phosphate Dibasic, 5.0 g

Potassium Phosphate Monobasic, 1.0 g

Magnesium Sulfate, 0.1 g

Tween 80, 1.0 ml

Dithiothreitol, 0.01 g

Vancomycin, 0.005 g

Josamycin, 0.003 g

Neomycin, 0.1 g

Sheep Blood, 50.0 ml

Distilled Water, 1000.0 ml

 

Final pH 7.4 +/- 0.2 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original container until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  90 days from date of manufacture.

 

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use:  FSA should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material.  Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37^oC for 18 – 48 hours.  Additional periods of incubation may be necessary to recover some anaerobes.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber / anaerobic jars, disinfectant, other culture media and serological and biochemical reagents.

 

Limitations

FSA will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  It is recommended that a non-selective medium such as Anaerobic Brucella Blood Agar also be inoculated from clinical specimens to assure growth of all species present.  Consult reference materials for additional information.

 

Quality Control

This medium should support good growth of most Fusobacterium species found in clinical infections.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.

Organism Tested	ATCC #	Results	Time (Hours)
Fusobacterium nucleatum	25586	Growth	24 – 48 h
Fusobacterium necrophorum	25286	Growth	24 – 48 h
Staphylococcus aureus	25923	No Growth
Prevotella melaninogenica	25845	No Growth
Bacteroides fragilis	25285	No Growth
Peptostreptococcus anaerobius	27337	No Growth
Clostridium perfringens	13124	No Growth
Clostridium difficile    or              Propionibacterium acnes	9689       6919	No Growth

 

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

 

Organism	Expected Growth
F. nucleatum	48 hrs
F. necrophorum	48 hrs
B. fragilis	Inhibited
C. perfringens	Inhibited

 

Physical Appearance:  FSA should appear opaque purple red in color.

References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974.  Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory Manual.  USDHEW C. D. C. Atlanta, GA 30333.

 

2.        Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R.  1992.  Principles of Practices of Clinical Anaerobic Bacteriology.  Star Publishing Co., Belmont, CA 94002.

 

3.        Holdeman, L. V., F. P. Cato and W. E. C. Moore.  1977.  Anaerobe Laboratory Manual.  Virginia Polytechnic Institute and State University.  Blacksburg, VA 24061.

 

4.        Somer-Jousimies, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold.  2002.  Wadsworth – KTL Anaerobic Bacteriology Manual.  Star Publishing Co., Belmont, CA 94002.

 

5.        Morgenstein, A. A., D. M. Citron and S. M. Finegold.  1981.  New Medium Selective for Fusobacterium Species and Differential for Fusobacterium necrophorum.  Journal of Clinical Microbiology.  Apr 1981 Vol. 13, No. 4.

 

6.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.