Formula
Bacto Proteose Peptone #3, 10.0 g
Bacto Beef Extract, 10.0
g
Yeast Extract, 5.0
g
Dextrose, 20.0
g
Sorbitan Monooleate Complex, 1.0 g
Ammonium Citrate, 2.0
g
Sodium Acetate, 5.0
g
Magnesium Sulfate, 0.1
g
Manganese Sulfate, 0.05
g
Potassium Phosphate, dibasic, 2.0 g
Bacto Agar, 15.0
g
Distilled Water, 1000.0
ml
Final pH 6.4 +/- 0.2 at 25 degrees C.
Final weight 16.0 g +/- 1.6.
Precautions
For IN VITRO DIAGNOSTIC USE only. Approved biohazard precautions and aseptic
techniques should be observed when using this product. This product is for use only by properly-trained
and qualified personnel. Sterilize all
biohazard waste prior to disposal.
Storage and Shelf Life
Storage: Upon receipt, store at room temperature (13°C - 27°C) in original packaging until use. Avoid overheating or freezing. Do not use medium if there are signs of
deterioration (shrinking, cracking or discoloration due to oxidation of media)
or contamination. The expiration date
applies to the product in its original packaging and stored as directed. Do not use product past the expiration date
shown on the container.
Shelf
Life: 90 days from date of manufacture.
Procedure
Specimen Collection:
Specimens for anaerobic culture should be protected from air (oxygen)
during collection, transport and processing of specimens. Consult appropriate references for detailed
instructions concerning collection and transport of anaerobes.
Methods for Use: LMRS
AGAR should be inoculated directly with clinical material. Inoculated plates should be immediately
placed in the appropriate atmosphere and incubated at 35 – 37oC for
18 – 48 hours. Additional periods of
incubation may be necessary to recover some anaerobes. Detailed instructions for processing
anaerobic cultures can be found in the appropriate references.
Materials Required But Not Provided
Standard
microbiological supplies and equipment such as loops, pipets, saline blanks,
slides, staining supplies, microscope, incinerator or autoclave, incubators,
anaerobic chamber or anaerobic jars, disinfectant, other culture media and
serological and biochemical reagents.
Limitations
LMRS AGAR will not provide complete information for the
identification of bacterial isolates.
Additional test procedures and media are required for complete
identification. Consult reference
materials for additional information.
Quality Control
If used properly, this medium should support good
growth of Lactobacillus from clinical
and non-clinical specimens.
The following organisms are routinely used for
quality assurance performance testing at Anaerobe Systems.
|
Organism Tested
|
ATCC #
|
Results
|
Time (Hours)
|
|
Lactobacillus vaginalis
|
49540
|
Growth
|
24 – 48 h
|
|
Lactobacillus acidophilus
|
4356
|
Growth
|
24 – 48 h
|
|
Lactobacillus crispatus
|
33197
|
Growth
|
24 – 48 h
|
|
Lactobacillus jensenii
|
25258
|
Growth
|
24 – 48 h
|
|
Lactobacillus fermentum
|
9338
|
Growth
|
24 – 48 h
|
|
Proteus mirabilis
|
12453
|
Growth
|
24 h
|
|
Staphylococcus
aureus or Enterococcus faecalis
|
25923
29212
|
Growth
|
24 h
|
|
Escherichia coli
|
25922
|
Growth
|
24 h
|
User
Quality Control: The final determination to the extent and quantity of user
laboratory quality control must be determined by the end user.
If
sterility testing is to be performed on this product, the appropriate
percentage of the original shipment amount should be incubated anaerobically
and aerobically for 48 – 96 hours.
If
the nutritive capacity of this medium is to be tested for performance, it is
recommended that the following ATCC
organisms be evaluated for growth.
|
Organism
|
Growth in
|
|
|
Hours
|
|
L. acidophilus
|
24-48 hrs
|
|
L. crispatus
|
24-48 hrs
|
|
L. fermentum
|
24-48 hrs
|
|
L. jensenii
|
24-48 hrs
|
|
S. aureus
|
24 hrs
|
Physical Appearance: LMRS AGAR should
appear as a translucent to opaque-yellowish color.
References
1.
Dowell, V. R., Jr. and T. M. Hawkins.
1974. Laboratory Methods in Anaerobic Bacteriology. CDC Laboratory Manual. USDHEW C. D. C. Atlanta, GA 30333.
2.
Holdeman, L. V., F. P. Cato and W. E. C. Moore. 1977. Anaerobe Laboratory Manual. Virginia Polytechnic Institute and State University. Blacksburg,
VA 24061.
3.
Engelkirk, P. G., Duben-Engelkirk, J. and Dowell, V. R. 1992. Principles and Practices of Clinical
Anaerobic Bacteriology. Star
Publishing Co., Belmont, CA 94002.
4.
Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J.,
Wexler, H. M. and S. M. Finegold.
2002. Wadsworth – KTL Anaerobic Bacteriology Manual. Star Publishing Co., Belmont, CA 94002.
5.
Isenberg, H. D. 1992. Clinical
Microbiology Procedures Handbook.
American Society for Microbiology Publishing, Washington, D.C. 20005.
6.
Murray, R. P., et al. 1999. Manual
of Clinical Microbiology. American
Society for Microbiology Publishing, Washington,
D.C. 20005.
7.
De Man, J. C., Rogosa, M. and Sharpe, M. E. 1960.
A medium for the cultivation of lactobacilli. J.
Appl. Bacteriol. 23(1), 130.
8.
NCCLS. Quality Control for Commercially Prepared Microbiological Culture
Media; Approved Standard- Third Edition.
(2004). NCCLS document
M22-A3. NCCLS, 940 West Valley Road, Suite 1400, Wayne,
PA 19087-1898.
