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Mitis Salivarius Agar with Tellurite (MSAT)

 
Mitis Salivarius Agar with Tellurite (MSAT) is intended for the selective isolation of Streptococcus mitis, Streptococcus salivarius, and other oral Streptococci and Enterococci found in clinical specimens.  MSAT is an enriched selective medium supplemented with Chapman Tellurite Solution for selection and isolation of S. mitis and S. salivarius.  Growth of S. mitis on the agar will appear as small or minute blue colonies, while S. salivarius produces blue smooth, gum drop, or rough colonies about 1-5 mm in diameter.  Enterococci produce a dark blue or black, shiny, slightly-raised colony on the agar.  This media is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

        Anaerobe Systems PRAS Mitis-Salivarius
               Agar w/ Tellurite (MSAT) AS-544


View the full product insert MSAT Insert.pdf

Mitis Salivarius Agar W/ Tellurite Products
 

 Item #

 Description

 Size

 Price

 AS-544

MSAT mono plate 

4 plates 

8.49

 




Formula

Mitis Salivarius Agar, 90.0 g

Chapman Tellurite Solution (1%), 1.0 ml

Distilled Water, 1000.0 ml

 

Final pH 7.3 +/- 0.3 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original packaging until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the package.

Shelf Life:  90 days from date of manufacture.

 

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use:  MSAT should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material.  Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37oC for 3-5 days.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

 

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber or anaerobic jars, other culture media and serological and biochemical reagents.

 

Limitations

MSAT will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  It is recommended that a non-selective medium, such as Anaerobic Brucella Blood Agar also be inoculated from clinical specimens to assure growth of all species present.  Consult reference materials for additional information.

 

Quality Control

MSAT should support good growth of Streptococcus mitis and Streptococcus salivarius from a variety of clinical specimens.

The following organisms are routinely used for quality control testing at Anaerobe Systems. 

Organism Tested

ATCC #

Results

Time (Hours)

Special Reaction

Bacteroides fragilis

25285

No Growth

48 h

 

Prevotella melaninogenica

25845

No Growth

48 h

 

Fusobacterium necrophorum

25286

No Growth

48 h

 

Fusobacterium nucleatum

25586

No Growth

48 h

 

Clostridium perfringens

13124

No Growth

48 h

 

Peptostreptococcus anaerobius

27337

No Growth

48 h

 

Enterococcus faecalis

29212

Growth

24-48 h

Blue/Black colonies

Staphylococcus aureus

25923

Inhibited

24-48 h

 

Streptococcus mitis

9811

Growth

24 h

Blue colonies

Streptococcus pyogenes

19615

Growth

24 h

Blue colonies

Streptococcus salivarius

13419

Growth

24 h

Blue “gum drop” colonies

Streptococcus mutans

25175

Growth

24-48 h

Blue colonies

  

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

 

Organism

Expected Growth

Special Reaction

F. nucleatum

No Growth

 

S. aureus

Inhibited

 

S. mitis

24 hrs

Blue colonies

S. pyogenes

24 hrs

Blue colonies

S. salivarius

24 hrs

Blue “gum drop” colonies

 

Physical Appearance:  MSAT should appear slightly opaque to translucent and royal blue in color.

 

References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory ManualUSDHEW C. D. C. Atlanta, GA 30333.

 

2.        Holdeman, L. V., F. P. Cato and W. E. C. Moore.  1977Anaerobe Laboratory ManualVirginia Polytechnic Institute and State University.  Blacksburg, VA 24061.

 

3.        Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold.  2002Wadsworth – KTL Anaerobic Bacteriology ManualStar Publishing Co., Belmont, CA 94002.

 

4.        Engelkirk, P. G., Duben-Engelkirk, J. and Dowell, V. R.  1992Principles and Practices of Clinical Anaerobic BacteriologyStar Publishing Co., Belmont, CA 94002.

 

5.        NCCLSQuality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition(2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

 

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