Anaerobe Systems PRAS Tryptic Soy Agar
     w/ N-Acetylmuramic Acid (TSA-NAM) AS-6421

 

View the full product insert: TSA-NAM Insert.pdf

Formula

Pancreactic Digest of Casein, 15.0 g

Agar, 15.0 g

Soy Peptone, 5.0 g

Sodium Chloride, 5.0 g

Hemin (0.1% Soln), 5.0 ml

N-acetylmuramic Acid, 10.0 mg

Vitamin K₁ (1% Soln), 1.0 ml

Sheep Blood, 50.0 ml

Distilled Water, 1000.0 ml

 

Final pH 7.2 +/- 0.2 at 25 degrees C.

Final weight 16.0 g +/- 1.6.

 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original container until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  90 days from date of manufacture.

 

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use:  TSA-NAM should be inoculated directly with clinical material or a broth that has been previously inoculated from clinical material.  Inoculated plates should be streaked to obtain isolated colonies, immediately placed in an anaerobic atmosphere and incubated at 35-37^oC for 48 – 72 hours.  Additional periods of incubation may be necessary to recover some anaerobes.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

 

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator / autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

 

Limitations

TSA-NAM will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  It is recommended that an enriched medium such as Anaerobic Brucella Blood Agar be inoculated from clinical specimens to assure growth of all species present.  Consult reference materials for additional information.

 

Quality Control

This medium should support good growth of Bacteroides forsythus found in clinical infections.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. 

Organism Tested	ATCC #	Results	Time (Hours)
Bacteroides fragilis	25285	Growth	24 h
Prevotella melaninogenica	25845	Growth	24-48 h
Fusobacterium nucleatum	25586	Growth	24-48 h
Clostridium perfringens	13124	Growth	24 h
Peptostreptococcus anaerobius	27337	Growth	24 h
Tannerella forsythensis	43037	Growth	48-72 h
Escherichia coli	25922	Growth	24 h

 

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth.

 

Organism	Expected Growth
B. fragilis	24 hrs
P. melaninogenica	24-48 hrs
F. nucleatum	24 hrs
C. perfringens	24 hrs
P. anaerobius	24 hrs
T. forsythensis	48-72 hrs

 

Physical Appearance:  TSA-NAM should appear opaque bright red in color.

 

References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974.  Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory Manual.  USDHEW C. D. C. Atlanta, GA 30333.

 

2.        Pamela H. Braham and Bernard J. Moncla.  1992.  Rapid Presumptive Identification and Further Characterization of Bacteroides forsythus.  Journal of Clinical Microbiology 30: 649-654.

 

3.    Holdeman, L. V., F. P. Cato, and W. E. C. Moore.  1977.  Anaerobe Laboratory Manual.  Virginia Polytechnic Institute and State University. Blacksburg, VA 24061.

 

4.        Engelkirk, P. G., Duben-Engelkirk, J. and Dowell, V. R.  1992.  Principles and Practices of Clinical Anaerobic Bacteriology.  Star Publishing Co., Belmont, CA 94002.

 

5.        Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and Finegold, S. M.  2002.  Wadsworth – KTL Anaerobic Bacteriology Manual.  Star Publishing Co., Belmont, CA 94002.

 

6.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.