Formula
Tryptic Soy Agar, 40.0
g
Yeast Extract, 1.0
g
Bacitracin, 75.0
mg
Vancomycin, 5.0
mg
Horse Serum, 100.0
ml
Distilled Water, 1000.0
ml
Final pH 7.1 +/- 0.2 at 25 degrees C.
Final weight 16.0 g +/- 1.6.
Precautions
For IN VITRO DIAGNOSTIC USE only. Approved biohazard precautions and aseptic
techniques should be observed when using this product. This product is for use only by properly-trained
and qualified personnel. Sterilize all
biohazard waste prior to disposal.
Storage and Shelf Life
Storage: Upon
receipt, store at room temperature (13°C - 27°C) in original container until
use. Avoid overheating or freezing. Do not use medium if there are signs of
deterioration (shrinking, cracking or discoloration due to oxidation of media)
or contamination. The expiration date
applies to the product in its original packaging and stored as directed. Do not use product past the expiration date
shown on the container.
Shelf
Life: 90 days from date of manufacture.
Procedure
Specimen Collection:
Specimens for anaerobic culture should be protected from air (oxygen)
during collection, transport and processing.
Consult appropriate references for detailed instructions concerning
collection and transport of anaerobes.
Methods for Use:
TSBV should be inoculated directly with clinical material or a broth
that has been previously inoculated from clinical material. Inoculated plates should be streaked to
obtain isolated colonies, immediately placed in an anaerobic atmosphere and
incubated at 35-37oC for 18 – 48 hours. Additional periods of incubation may be
necessary to recover some anaerobes.
Detailed instructions for processing anaerobic cultures can be found in
the appropriate references.
Materials Required But Not Provided
Standard
microbiological supplies and equipment such as loops, saline blanks, slides,
staining supplies, microscope, incinerator / autoclave, incubators, anaerobic
chamber, other culture media and serological and biochemical reagents.
Limitations
TSBV will not provide complete information for
identification of bacterial isolates.
Additional test procedures and media are required for complete identification. It is recommended that a non-selective medium
such as Anaerobic Brucella Blood Agar also be inoculated from clinical
specimens to assure growth of all species present. Consult reference materials for additional
information.
Quality Control
This medium should support good growth of A. actinomycetemcomitans found in
clinical infections.
The following organisms are routinely used for
quality assurance performance testing at Anaerobe Systems.
|
Organism Tested
|
ATCC #
|
Results
|
Time (Hours)
|
|
Actinobacillus actinomycetemcomitans
|
29522
|
Growth
|
24 – 48 h
|
|
Actinomyces viscosus
|
43146
|
No Growth
|
|
|
Fusobacterium nucleatum
|
25586
|
Growth
|
24 – 48 h
|
|
Propionibacterum acnes
|
9619
|
No Growth
|
|
|
Clostridium difficile
|
9689
|
No Growth
|
|
|
Enterococcus faecalis
|
29212
|
No Growth
|
|
User
Quality Control: The final determination to the extent and quantity of user
laboratory quality control must be determined by the end user.
If
sterility testing is to be performed on this product, the appropriate
percentage of the original shipment amount should be incubated anaerobically
and aerobically for 48 – 96 hours.
If
the nutritive/inhibitory capacity of this medium is to be tested for
performance, it is recommended that the following ATCC
organisms be evaluated for growth/inhibition.
|
Organism
|
Expected Growth
|
|
A. actinomycetemcomitans
|
24-48 hrs
|
|
A. viscosus
|
Inhibited
|
|
F. nucleatum
|
24-48 hrs
|
|
E. faecalis
|
Inhibited
|
Physical Appearance: TSBV should appear translucent yellow in
color.
References
1.
Dowell, V. R., Jr. and T. M. Hawkins.
1974. Laboratory Methods in Anaerobic Bacteriology. CDC
Laboratory Manual. USDHEW C. D. C.
Atlanta, GA 30333.
2.
Engelkirk, P. G., Duben-Engelkirk, J., Dowell, V. R. 1992. Principles of Practices of Clinical
Anaerobic Bacteriology. Star
Publishing Co., Belmont, CA 94002.
3.
Holdeman, L. V., F. P. Cato and W. E. C. Moore. 1977. Anaerobe Laboratory Manual. Virginia Polytechnic Institute and State University. Blacksburg,
VA 24061.
4.
Slots, J. 1982. Selective Medium for Isolation of Actinobacillus actinomycetemcomitans. Journal
of Clinical Microbiology. April
1982, p. 606 – 609.
5.
Somer-Jousimies, H. R., Summanen, P., Citron, D. M., Baron, E. J.,
Wexler, H. M. and S. M. Finegold.
2002. Wadsworth – KTL Anaerobic Bacteriology Manual. Star Publishing Co., Belmont, CA 94002.
6.
NCCLS. Quality Control for Commercially Prepared Microbiological Culture
Media; Approved Standard- Third Edition.
(2004). NCCLS document
M22-A3. NCCLS, 940 West Valley Road, Suite 1400, Wayne,
PA 19087-1898.
