Anaerobe Systems PRAS Brain Heart
             Infusion Broth (BHI) AS-872

Formula

Brain Heart Infusion, 37.0 g

                Calf Brain Infusion, 7.7 g

                Beef Heart Infusion, 9.8 g

                Proteose Peptone, 10.0 g

                Dextrose, 2.0 g

                Sodium Chloride, 5.0 g

                Disodium Phosphate, 2.5 g

Hemin, .005 g

Vitamin K_1, .002 g

Resazurin, .001 g

Yeast Extract, 5.0 g

L-Cysteine, 0.5 g

Distilled Water, 1000.0 ml

 

Final pH 7.3 +/- 0.3 at 25 degrees C.

Final volume 5.0 ml +/- 0.5.

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original packaging until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (discoloration or evaporation) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  1 year from date of manufacture.

 

Procedure

Specimen Collection:  Specimens for anaerobic culture should be protected from air (oxygen) during collection, transport and processing of specimens.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use:  BHI should be inoculated directly with clinical material.  Inoculated tubes should be immediately placed in an anaerobic atmosphere and incubated at 35 – 37^oC for 18 – 48 hours.  Additional periods of incubation may be necessary to recover some anaerobes.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

 

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, pipets, saline blanks, slides, staining supplies, microscope, incinerator/autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

 

Limitations

BHI will not provide complete information for the identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  Consult reference materials for additional information.

 

Quality Control

If used properly, this medium should support good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems. 

Organism Tested	ATCC #	Results	Time (Hours)
Bacteroides fragilis	25285	Growth	24 h
Prevotella melaninogenica	25845	Growth	24 – 48 h
Bacteroides vulgatus	8482	Growth	24 h
Fusobacterium nucleatum	25586	Growth	24 – 48 h
Fusobacterium necrophorum	25286	Growth	24 – 48 h
Clostridium perfringens	13124	Growth	24 h
Clostridium novyi	7659	Growth	24 – 48 h
Peptostreptococcus anaerobius	27337	Growth	24 h
Clostridium difficile	9689	Growth	24 h
Staphylococcus aureus	25923	Growth	24 h

 

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive capacity of this media is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth.

 

Organism	Expected
	Growth
B. fragilis	24 hrs
P. melaninogenica	48 hrs
C. perfringens	24 hrs
F. necrophorum	48 hrs
S. aureus	24 hrs

 

Physical Appearance:  BHI should appear as a clear, yellowish liquid within the 16mm x 100mm glass tube.

 

 References

1.        Dowell, V. R., Jr. and T. M. Hawkins.  1974.  Laboratory Methods in Anaerobic Bacteriology.  CDC Laboratory Manual.  USDHEW C. D. C. Atlanta, GA 30333.

 

2.        Dowell, V. R., Jr. and G. L. Lombard.  1977.  Presumptive Identification of Anaerobic Non-sporeforming Gram-negative Bacilli.  USDHEW, CDC.  Atlanta, GA 30333.

 

3.        Holdeman, L. V., F. P. Cato and W. E. C. Moore.  1977.  Anaerobe Laboratory Manual.  Virginia Polytechnic Institute and State University.  Blacksburg, VA 24061.

 

4.        Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. and S. M. Finegold.  2002.  Wadsworth – KTL Anaerobic Bacteriology Manual.  Star Publishing Co., Belmont, CA 94002.

 

5.        Wilkins, T. D. and T. Theil.  1973.  Modified Broth-Disc Method for Testing the Antibiotic Susceptibility of Anaerobic Bacteria.  Antimicrob. Agents Chemother. 3: 350 – 356.

 

6.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.

 

7.        Engelkirk, P. G., Duben-Engelkirk, J. and Dowell, V. R.  1992.  Principles and Practices of Clinical Anaerobic Bacteriology.  Star Publishing Co., Belmont, CA 94002.