Formula

Pancreatic Digest of Casein, 15.0 g

Yeast Extract, 5.0 g

Animal Tissue, 3.0 g

Sodium Chloride, 2.5 g

Dextrose, 6.0 g

Sodium Thioglycolate, 0.5 g

Agar, 0.7 g

Sodium Sulfite, 0.1 g

L-Cystine, 0.25 g

Hemin (0.1% soln), 5.0 ml

Vitamin K₁ (1% soln), 0.1 ml

Vancomycin, 10.0 mg

Trimethoprim, 5.0 mg

Polymyxin B, 5000 I.U.

Amphotericin B, 3.55 mg

Marble Chip, 1 piece each

Distilled Water, 1000.0 ml

 

Final pH 7.6 +/- 0.3 at 25 degrees C.

Final volume 7.0 mL +/- 0.7.

 

Precautions

For IN VITRO DIAGNOSTIC USE only.  Approved biohazard precautions and aseptic techniques should be observed when using this product.  This product is for use only by properly-trained and qualified personnel.  Sterilize all biohazard waste prior to disposal.

 

Storage and Shelf Life

Storage:  Upon receipt, store at room temperature (13°C - 27°C) in original packaging until use.  Avoid overheating or freezing.  Do not use medium if there are signs of deterioration (discoloration or evaporation) or contamination.  The expiration date applies to the product in its original packaging and stored as directed.  Do not use product past the expiration date shown on the container.

Shelf Life:  1 year from date of manufacture.

 

Procedure

Specimen Collection:  It is important to maintain the viability of Campylobacter species during transport, storage and processing of specimens.  Consult appropriate references for detailed instructions concerning collection and transport of anaerobes.

Methods for Use:  CAMPY-THIO media should be inoculated directly from a clinical specimen (i.e. stool sample).  The tube is then incubated at 4°C for 8 – 12 hours.  After refrigeration, a sample from the tube is streaked onto appropriate Campylobacter media and a Brucella media for further isolation of organisms.  Incubate inoculated plates at 42 – 43^o C in an anaerobic jar (catalyst removed) with a microaerophilic gas mixture.  For strain specific gas mixtures, consult appropriate references. 

NOTE:  If facilities for gassing out an anaerobic jar are not available, a disposable Hydrogen-Carbon Dioxide generator may be substituted.  Not all strains of Campylobacter jejuni subsp. jejuni grow as well when generators are used, and some may not grow at all.  Campylobacter jejuni subsp. jejuni is a microaerophile, not a strict anaerobe.

Plates should be examined after 24, 48 and 72 hours of incubation.  Colonies of Campylobacter jejuni subsp. jejuni are usually detected in 24 hours.  The colonies vary from pinpoint, glossy-appearing to those which spread over the entire surface of the agar.  Since Campylobacter jejuni subsp. jejuni is an oxidase positive organism, the oxidase test can be used to screen suspect colonies.

The cold enrichment step with CAMPY-THIO has been shown to increase the recovery of Campylobacter jejuni subsp. jejuni from stool samples (1, 3).  Overgrowth of other organisms should be inhibited.  Detailed instructions for processing anaerobic cultures can be found in the appropriate references.

 

Materials Required But Not Provided

Standard microbiological supplies and equipment such as loops, saline blanks, slides, staining supplies, microscope, incinerator/autoclave, incubators, anaerobic chamber, other culture media and serological and biochemical reagents.

 

Limitations

CAMPY-THIO medium will not provide complete information for identification of bacterial isolates.  Additional test procedures and media are required for complete identification.  Some organisms normally recovered from this medium may be inhibited.  Consult reference materials for additional information.

 

Quality Control

If used properly, this medium should aide in the isolation of Campylobacter species from clinical material contaminated by normal flora.  Campylobacter jejuni subsp. jejuni, and Campylobacter fetus subsp. fetus should be recovered upon subculturing onto appropriate media.

The following organisms are routinely used for quality assurance testing at Anaerobe Systems. 

Organism Tested	ATCC #	Results	Time (Hours)
Campylobacter jejuni subsp. jejuni	33290	Growth	24 – 48 h
Campylobacter fetus subsp. fetus	33246	Growth	24 – 48 h
Staphylococcus aureus	25923	No Growth
Enterococcus faecalis	29212	No Growth
Escherichia coli	25922	No Growth
Proteus mirabilis	12453	Inhibited to No Growth

 

User Quality Control: The final determination to the extent and quantity of user laboratory quality control must be determined by the end user.

If sterility testing is to be performed on this product, the appropriate percentage of the original shipment amount should be incubated anaerobically and aerobically for 48 – 96 hours.

If the nutritive/inhibitory capacity of this medium is to be tested for performance, it is recommended that the following ATCC organisms be evaluated for growth/inhibition.

 

Organism	Growth in
	Hours
C. jejuni subsp. jejuni	48 hrs
C. fetus subsp. fetus	48 hrs
S. aureus	Inhibited
E. coli	Inhibited

 

Physical Appearance: CAMPY-THIO is a clear, golden-yellow liquid within the tube with a submerged marble chip in a 16mm x 100mm glass tube.

References

1.    Kaplan, R. L. and J. E. Barret.  1981.  Monograph:  Campylobacter.  Marion Scientific.

 

2.        Lennette, E. H., et al.  1980.  Manual of Clinical Microbiology (3^rd edition), American Society for Microbiology.  Washington D. C.

 

3.        Rubin, S. J. and M. Woodward.  1983.  Enhanced isolation of Campylobacter jejuni by cold enrichment in Campy-Thio broth.  J. Clin. Microbiol. 18:  1008-1010.

 

4.        Wang, W. L. et. Al.  1983.  Evaluation of transport media for Campylobacter jejuni in human fecal specimens.  J. Clin. Microbiol.  18:  803-807.

 

5.        NCCLS.  Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard- Third Edition.  (2004).  NCCLS document M22-A3.  NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898.